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EFFECTS OF PHLEBOTOMY IN PATIENTS WITH CHRONIC HEPATITIS C WHO FAILED TO RESPOND TO A PRIOR COURSE OF INTERFERON [alpha]-2B.
Author: JL Herrera. Division of Gastroenterology, University of South Alabama College of Medicine, Mobile, AL.

To assess if phlebotomy enhances the response to interferon (IFN) in patients with chronic hepatitis C, we prospectively evaluated 14 patients who had failed (n=11) or relapsed (n=3) after a prior course of interferon [alpha]-2b. All patients underwent weekly phlebotomy until serum ferritin was less than lower limits of normal. Patients then received interferon [alpha]-2b, 3 M.U. 3X/wk for 24 weeks. No additional phlebotomies were done during interferon therapy. Serum ALT, ferritin and HCV RNA by PCR and bDNA were measured at baseline, pre-interferon (after phlebotomy) and at 12 & 24 wks of interferon therapy. Response to treatment was defined as HCV-RNA PCR negative at the end of treatment. Fourteen patients, mean age (±SEM) 48±3 yr. (10 M, 4F) were studied. Liver histology showed mild chronic hepatitis. in 2, severe chronic hepatitis in 8 and cirrhosis in 4.
Laboratory evaluation:

  Baseline     Pre-IFN          WK 12       WK 24 IFN
                             (post-phlebotomy) IFN
  ALT (U/L)      152±24       99±14            79±14        115±25
  FERRITIN       241±80       11±2             20±4          27±9(ng/ml)
  HCVRNA         77±18        50±10            30±10         17±4(x10Ø{5})
  All values are expressed as mean ± SEM

Significant decrease in ALT (p=0.004) and bDNA levels (p=0.009) were noted after phlebotomy. No further significant improvement in ALT was noted on interferon (p=0.45). Although interferon therapy resulted in further significant decrease in viremia (p=0.04 pre IFN vs. wk 24 IFN), all patients were HCV-RNA PCR positive at the end of therapy.

CONCLUSION: Iron deficiency induced by phlebotomy does not result on viral eradication during interferon therapy in patients who had previously failed a course of interferon. This research was funded in part by Schering-Plough, Kenilworth, New Jersey.

Source: American Association for the Study of Liver Diseases - 1996 Annual Meeting


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