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Phyllanthus Fraternus - A Plant with Anti-Hepatitis Viral
Activity
Aqueous extracts of Phyllanthus fraternus (used in Ayurveda and
Siddha systems of medicine for the treatment of jaundice) possesses
anti-hepatitis virus DNA polymerase activity. As the availability
of P. fraternus is restricted by geographical distribution and
seasonal growth, the present investigations were aimed at
standardizing procedures for micropropagation of P. fraternus using
shoot tip and nodal cultures to make it available all-year-round.
Shoot tip and nodal explants were cultured on B-5 medium
supplemented with 0, 10(- 7), 10(-6) and 10(-5) M benzylaminopurine
(BAP) or kinetin (KN). The presence of BAP in the medium promoted
multiple shoot formation from both types of explants. Of the two
explants, shoot tips were found to be more amenable for shoot
multiplication. About 14-16 shoots were obtained from a single
shoot tip in approximately 28 days on medium with 10(-5) M BAP.
Whenever required the individual shoots could be rooted on
half-strength B, medium supplemented with 10(-6) M IBA. Thus the
media standardised for multiple shoot formation/axillary bud
sprouting through shoot tip cultures can be exploited for
multiplication of desirable genotypes or potential somaclonal
variants (such as those with high levels of active principle or
with higher therapeutical value) at commercial level. Moreover,
these can be directly used for drug production without any chemical
pretreatments. (C) 1997 Elsevier Science B.V.
Author: PP SARADHI, JAMIA MILLIA ISLAMIA, CTR BIOSCI, NEW
DELHI 110025, INDIA Source: INDUSTRIAL CROPS AND PRODUCTS 1997
FEB;6(1):35-40
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