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Comparison of quantitative HCV RNA assays in chronic hepatitis
C
Author: Jacob S, Baudy D, Jones E, Xu L, Mason A, Regenstein
F, Perrillo RP Section of Gastroenterology and Hepatology, Ochsner
Clinic, New Orleans, Louisiana, USA.
Source: Am J Clin Pathol 107: 362-367 (1997)
We compared the relative sensitivities of first-and-second
generation branched nucleotide assays (Quantiplex HCV RNA 1.0 and
2.0, respectively, Chiron, Emeryville, Calif) for the detection of
hepatitis C virus (HCV) RNA to that of a commercially available
quantitative reverse transcriptase polymerase chain reaction
(RT-PCR) method (Monitor, Roche Molecular Systems, Nutley, NJ) in
53 patients with chronic hepatitis C. The sensitivities of the
second-generation branched DNA (bDNA) and RT-PCR assays were
similar (91% and 92%, respectively), and both were significantly
more sensitive (P < .001) than the first-generation method.
Moreover, both assays detected HCV RNA in all 11 patients with type
2a, 2b, or 3a genotypes vs 45% with the HCV RNA 1.0 bDNA assay. We
examined 174 serum samples by the bDNA 2.0 and RT-PCR assays. Major
quantification differences were noted on a given specimen with the
RT-PCR method reporting values an average 41-fold lower (range,
0-703-fold) than those obtained with the bDNA assay. We conclude
that both methods can be used to detect HCV RNA in patients who are
infected with the genotypes that are most commonly encountered in
the United States. The HCV RNA 2.0 bDNA assay may offer advantages
when attempting to quantify high-level viremia.
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