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TITLE: Polymerase Chain-Reaction For The Detection Of Hcv-Rna -
Cryoglobulinemia As A Cause For False-Negative Results
Polymerase chain reaction is widely used in clinical practice as
a reliable assay for the detection and quantitation of hepatitis C
virus RNA in serum and tissue. Due to the high sensitivity of the
test, both false positive and false negative results can occur The
presence of cryoglobulins in serum samples represents a variable
that can influence the result of a polymerase chain reaction assay
lending to false negative results. In cases of hepatitis C virus
infection associated with cryoglobulinaemia, it is extremely
important to very carefully process the samples to be tested in
order to avoid cryoglobulin precipitation and the consequent
removal of the virus from the sample. AUTHOR: DH VANTHIEL, UNIV
KENTUCKY, ALBERT B CHANDLER MED CTR, DEPT GASTROENTEROL and NUTR,
LEXINGTON, KY 40536
SOURCE: ITALIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 1997
JUN;29(3):273-274
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