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TITLE: A method for the detection and confirmation of
antibodies to hepatitis C virus in dried blood spots.
This study describes the development and evaluation of a cost
effective test rationale for the detection of anti-HCV in dried
blood spots. Samples were screened using an 'in house' IgG ELISA
that incorporated the recombinant proteins c22-3, c200 and NS5.
Confirmation of specific antibody to HCV was by a modification of
the immunoblot RIBA 3.0. An extensive panel of well evaluated
anti-HCV positive and negative samples from the UK and South Africa
were used to assess the sensitivity and specificity of the two
tests. One third of the anti-HCV positive samples had been typed.
All anti-HCV positive samples were detected by the 'in house'
screening EIA. Test/negative optical density ratios showed that
more than 95% of reactive samples produced values greater than 5.0.
Antibodies to HCV could be detected in a wide range of samples
derived from asymptomatic and symptomatic patients and of different
genotypes, with similar sensitivity. The presence of anti-HCV could
be confirmed by RIBA 3.0 in samples with low reactivity but not in
anti-HCV negative samples. Furthermore the immunoblot assay
successfully increased specificity by screening out false reactive
EIA samples that might occur in an epidemiological survey of a
multi-ethnic population.
AUTHOR: Parker SP, Cubitt WD, Ades AE; SOURCE: J Virol
Methods 1997 Nov;68(2):199-205
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